For the past five years, Professor Cain’s research team has been developing a new technique called “Histotripsy” which is a non-invasive ultrasonic tissue fractionation approach for the treatment of benign disease and cancer. Histotripsy results in localized tissue removal with sharp boundaries, which they use to remove cardiac tissue in treatment of congenital heart disease. In bulk tissue, histotripsy produces mechanical fragmentation of tissue resulting in a liquefied core with very sharply demarcated boundaries. Histology demonstrates that treated tissue within the lesion is fragmented to subcelluar level surrounded by an almost imperceptibly narrow margin of cellular injury. Histotripsy has vast clinical applications where precise tissue ablation and removal are needed (e.g., tumor treatment).